The 52-year-old will appear at Westminster Magistrates' Court later today accused of murder and three other offences.
Thomas Mair, 52, has been charged with murder in connection with the shooting of Labour MP Jo Cox, West Yorkshire Police said.
Mrs Cox was attacked in the street outside her constituency surgery in Birstall, near Leeds, at lunchtime on Thursday.
West Yorkshire Police say Mair has also been charged with grievous bodily harm, possession of a firearm with intent to commit an indictable offence and possession of an offensive weapon.
He will appear at Westminster Magistrates' Court later today.
Temporary Chief Constable Dee Collins said that a 77-year-old man is in a stable condition in hospital after being injured trying to help Mrs Cox.
Vigils have been held across the UK to remember Mrs Cox, who came to politics from a career in aid work.
The Labour MP won the seat of Batley and Spen in last year's general election with a majority of more than 6,000 votes.
The Conservative Party and the Liberal Democrats have said they will not contest the by-election resulting from her death, allowing Labour a free run at keeping the seat.
Tributes have also come from world leaders, with US President Barack Obama even calling Mrs Cox's husband Brendan from Air Force One to express his condolences on behalf of the American people.
UK Labour Party leader Jeremy Corbyn described her as "an exceptional, wonderful, very talented woman, taken from us in her early 40s when she had so much to give and so much of her life ahead of her".
And London Mayor Sadiq Khan called her "the best of politics", telling Sky News that the way British politics is conducted is "poisonous" and that there needs to be a change of attitude.
More than £219,000 had been raised by the early hours of Saturday on a fundraising page set up by friends of Mrs Cox to support three charities "closest to her heart".
The Remain and Vote Leave sides suspended national campaigning ahead of next week's EU Referendum out of respect for Mrs Cox.
The National Police Chiefs' Council said forces would be contacting MPs to give security advice, as many MPs said they would go ahead with constituency surgeries, despite the killing.
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